Composite

Part:BBa_K3560004:Design

Designed by: ShiDi Xiao   Group: iGEM20_XHD-Wuhan-China   (2020-10-23)


PGroES-DrRBS-gcd


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal AgeI site found at 1797
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Theoretically, after PGroES-DrRBS-gcd is transformed to Deinococcus radiodurans, Deinococcus radiodurans can secrete gluconic acid, gradually decrease the pH of the solution, and promote the dissolution of phosphate.


Source

we recombined the gcd gene from E. coli and the Deinococcus radiodurans shuttle plasmid pRADK to form a gcd-pRADK recombinant plasmid, constructed PGroES-DrRBS-gcd.

References